Next, we examined the burden of driver mutations that were defined as non-silent SNVs and short insertions and deletions (indels) with high MAF in cancer-driver genes (ARID1A, CTNNB1, FBXW7, KRAS, PIK3CA, PIK3R1, PPP2R1A, PTEN, and TP53). This evidence concerns the gene PIK3CA and cancer.