IMMT and glioblastoma: Therefore, we sought to reproduce the phenotype of Mic60-low tumors (Fig. 1A and SI Appendix, Fig. S1 A–C) by generating clones of PC3 or GBM LN229 cells with silencing of Mic60 by short hairpin RNA (shRNA) or CRISPR-Cas9 (SI Appendix, Fig. S2A, Top).