In the present study, the total white blood cell count in BALF and the concentrations of IL-8 and TNF-α in BALF and pulmonary tissues were significantly higher, while the concentration of IL-10 was significantly lower, in groups M, N, and D than in group C, suggesting the existence of an inflammatory response in the pulmonary tissues in the COPD rat model. Here, TNF is linked to chronic obstructive pulmonary disease.