For example, filtering on LOEUF excluded all variants in TP53 and a D90A variant in SOD1 associated with incompletely penetrant amyotrophic lateral sclerosis (Al-Chalabi et al., 1998) that may be relevant to groups modeling neurodegenerative disease, and our sequencing quality filters removed two of the five variants previously identified in TP53 since they were present at low allelic fractions. Here, SOD1 is linked to neurodegenerative disease.