Similar to SK-N-SH neuroblastoma cells and MHCC-LM3 liver cancer cells (14), the B10F10 melanoma cells also expressed agrn at a similar level compared to that of the skeletal muscle ECs of EC-agrn+/+ mice but at a significantly lower level than that of the skeletal muscle ECs of EC-agrnfl/fl mice (Figure 4K), suggesting that tumor angiogenesis could be rescued by tumor derived agrin even in endothelial agrin deficient mice. Here, AGRN is linked to melanoma.