Finally Gonzalez-Rodriguez et al. studied liver samples from healthy control, NAFL, and NASH patients and established that patients with NASH had significantly less autophagy than both other groups as seen by their increased p62 protein levels, increased phopspho-mTOR, increased phospho-S6K1, and decreased mRNA levels of Beclin-1 (Gonzalez-Rodriguez et al., 2014). The gene discussed is RPS6KB1; the disease is metabolic dysfunction-associated steatohepatitis.