Additionally, the prediction accuracy of FRGPI was highly stable in different clinical character groups, including <45 and >45-year age group, LGG (grade I–II gliomas are classified as LGG in the TCGA database) and GBM (grade IV gliomas) groups, IDH1-WT and IDH1-Mut groups, 1p19q non-codel group, except in 1p19q codel group, in TCGA training set (Supplementary Figures 5A–H), and these results were verified again in the CGGA validation set (Supplementary Figures 5I–P). This evidence concerns the gene IDH1 and glioblastoma.