When the translation of ARHGEF3 was blocked with MO antisense oligonucleotides at an early stage, the ARHGEF3 MO-injected embryos showed normal morphologic development but evident defects in hematopoiesis, especially with microcytic and hypochromic anemia (Serbanovic-Canic et al., 2011). This evidence concerns the gene ARHGEF3 and hypochromic anemia.