Based on the expression profiles of ASNS, CEBPA, and CAD, 415 TCGA colon cancer samples were analyzed by consistent clustering (Figure 2B) and tSNE algorithm (Figure 2C), and the samples were divided into two clusters, Cluster 1 and Cluster 2, with longer and shorter survival time, respectively. The gene discussed is ASNS; the disease is malignant colon neoplasm.