CEBPA was the upstream gene of ASNS, and the expression of CEBPA and ASNS is positively correlated.We predict that upstream factors such as CREB, insulin, and RNA Pol II can regulate downstream genes, such as ASNS and SLC7A11, by affecting the expression of CEBPA, ultimately changing the cellular function and metabolism in colon cancer tissues. Here, ASNS is linked to malignant colon neoplasm.