Gene set variation analysis (Figure 3A; Supplementary Table 3) revealed that ToF and ASD RA samples showed a significant difference in the metabolism-related pathways of lipid droplet (p = 0.011), lipid binding (p = 0.0037), carbohydrate metabolic process (p = 3.23E−06), and heme metabolism (p = 0.011). This evidence concerns the gene GATA4 and atrial septal defect.