TSPAN8 and rheumatoid arthritis: In this study, we demonstrated screening of a bacterial antigen (tetanus toxoid), an autoantigen linked to Rheumatoid Arthritis (Glucose-6-phosphate Isomerase), and an insoluble, membrane-expressed antigen (tetraspanin-8; TSPAN8); antigen-specific ASC against all 3 model antigens were able to be sorted by this flowed droplet microfluidic technique.