CD4 and hepatocellular carcinoma: Further comparison of ICIs on CD4 and CD8 T cells in livers of HCC-bearing and HCC-free mice independent on vaccination strategy revealed a significant upregulation of CD160, TIM-3, and PD-1 molecules on CD4 and CD8 T lymphocytes in HCC-Ova-harboring livers (Supplementary Fig. S8A, B, gating strategy shown in Supplementary Fig. S8C).