Double-deleted vaccine strain LmAI has several important advantages: it can efficiently deliver large tumor antigens; it can be efficiently taken up by antigen-presenting cells, thereby inducing potent specific CD4/CD8 T cell responses due to the expression of important virulent factors like listeriolysin O [15, 16]; it is safe even in advanced cancers as previously reported [10, 15, 16] and also confirmed in our study. This evidence concerns the gene CD4 and cancer.