Compared with those in the normal group, the levels of ALT (135.27 ± 19.32 U/L), AST (178.64 ± 21.87 U/L), hepatic IL-1β (254.06 ± 29.82 pg/g liver), IL-6 (326.93 ± 42.55 pg/g liver), and TNF-α (231.24 ± 32.66 pg/g liver) increased significantly in the NASH rats. This evidence concerns the gene IL6 and metabolic dysfunction-associated steatohepatitis.