The present experimental results demonstrated that MCD-induced rats showed increased levels of hepatocellular IL-1β, IL-6, and TNF-α, which might suggest a potential mechanism for the down-regulation of the Oatplal, Oatp1a4, and Oatp1b2 expressions in these animals accompanied by the NASH. This evidence concerns the gene IL1B and metabolic dysfunction-associated steatohepatitis.