We further analyzed the relationship among DIRAS3 protein expression and age, gender, pathological grade, pathological differentiation, TNM stage, and lymph node metastasis (LNM) of NSCLC patients, and as illustrated in Table 1, we uncovered that DIRAS3 protein expression was associated with pathological differentiation, TNM stage, and LNM. Here, DIRAS3 is linked to metastatic malignant neoplasm in the lymph nodes.