In addition, as a RhoA/ROCK downstream signaling factor, activation of myosin light chain 2 (MLC2) was produced in the form of phosphorylation, and papers have demonstrated the involvement of RhoA/ROCK/MLC2 in cerebral ischemia-reperfusion injury to the blood-brain barrier [21–23]. Here, MYL2 is linked to Cerebral ischemia.