Previously, we isolated CCSCs from two CRC patients using CCSC markers ALDH1, CD133, and CD44, and characterized the stem‐like features by the sphere propagation assay and limiting dilution assay.[10] We then co‐cultured the CCSC sphere cells with F. nucleatum to determine whether F. nucleatum regulates CCSC self‐renewal. The gene discussed is ALDH1A1; the disease is colorectal carcinoma.