The UAP1L1 overexpression, CDCA8 knockdown and UAP1L1 overexpression accompanied by CDCA8 knockdown cell models were constructed successfully in vitro by lentivirus transfection in order to investigate the effects of CDCA8 regulated by UAP1L1 on prostate cancer cells (Additional file 1: Fig. S3A). Here, UAP1L1 is linked to Familial prostate cancer.