In conclusion, through sequencing, it was found that ICMT, OGDH, TKT, ZMPSTE24, RPS6KA1, CACNA1B, and MAPK8IP3, as well as pathways for biosynthesis of antibiotics, terpenoid backbone biosynthesis, and MAPK signaling pathway may play important roles in osimertinib resistance in NSCLC. The gene discussed is RPS6KA1; the disease is non-small cell lung carcinoma.