Then, PI3K inhibitor LY294002 and CDK1 interference RNA methods were employed to explore the effect of EPO on the PI3K-IKKα-CDK1 signaling pathway of U251 cell glioma line under hypoxia, so as to an provide experimental basis for studying the neuroprotective mechanism of EPO in brain injury caused by neonatal asphyxia. This evidence concerns the gene CHUK and glioma.