CDH1 and renal fibrosis: We observed that TGF-β1 stimulated upregulation of α-SMA and accompanied by downregulation of E-Cad (Figure 4a,4g,4h), indicating that TGF-β1 initiated renal fibrosis via epithelial mesenchymal transition (EMT) in HK-2 cells, which was in accordance with previous reports 40, 41.