METTL3 and inflammatory bowel disease: Since METTL3 is up-regulated in IBD, METTL3 knockdown was achieved in mouse intestinal epithelial cell line MODE-K via transfecting lentivirus containing specific short hairpin RNA (#1 sh-METTL3 or #2 sh-METTL3) and confirmed using qRT-PCR and Immunoblotting (Fig. 2A, B).