We found that HCC patients with lower HPN levels in enriched B cells (P = 2.1e−006), CD4 + memory T cells (P = 8.8e−03), CD8 + T cells (P = 8e−03), natural killer T cells (p = 5.8e−03), Treg T cells (p = 3.5e−03), type 1 T helper cells (P = 4.7e−03), type 2 T-helper cells (P = 3.5e−03) cohort were more likely to have a worse prognosis (Fig. 7a–h). This evidence concerns the gene CD8A and hepatocellular carcinoma.