SiRNA targeting RAB27B or siRNA-NC (5′- CAGUAGGAAUAGACUUUCG dTdT-3′, 3′-dTdTGUCAUCCUUAUCUGAAAGC-5′), BDH2 overexpressing plasmids or empty plasmid (PcDNA3.1) were constructed and purchased from Genomeditech (Shanghai, China) transfected into AML cell using LipofectamineTM2000 with the manufacturer’s instructions (Invitrogen, USA). The gene discussed is RAB27B; the disease is acute myeloid leukemia.