SiRNA targeting RAB27B or siRNA-NC (5′- CAGUAGGAAUAGACUUUCG dTdT-3′, 3′-dTdTGUCAUCCUUAUCUGAAAGC-5′), BDH2 overexpressing plasmids or empty plasmid (PcDNA3.1) were constructed and purchased from Genomeditech (Shanghai, China) transfected into AML cell using LipofectamineTM2000 with the manufacturer’s instructions (Invitrogen, USA). Here, BDH2 is linked to acute myeloid leukemia.