Western blotting (Figure 2b) and statistical analysis (Figure 2c-e) results indicated that the expression of RIP1, RIP3 and MLKL was higher in the CON+IR group than in the CON group (Figure 2c,P < 0.01; Figure 2d, P < 0.01; Figure 2e, P < 0.001), thus confirming that necroptosis in AKI occurred and was induced by IR. Here, MLKL is linked to acute kidney injury.