Complications in diabetes can be regulated by PKC at multiple levels such as via activation of eNOS, NAD(P)H oxidase, phospholipase A2 (PLA2), endothelin-1 (ET-1), vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and NF-κB. The gene discussed is EDN1; the disease is diabetes mellitus.