CASP8 and cancer: Similarly, the anti-cancer potential of B. leachii ink concentrate at 100 and 400 μg/mL through apoptosis induction in HepG2 cells treated for 24 h was confirmed using Western blot technology by quantitatively detecting the expression of the most important pro-apoptotic proteins, namely the initiator caspase-8 and the effector/executioner caspase 3.