In patients with moderate-to-severe inflammation, we expect that the TSAT/hepcidin ratio has a lower specificity to distinguish IRIDA from TMPRSS6-unrelated IDA, as inflammation has been demonstrated to increase hepcidin synthesis and lower TSAT levels [8], which might result in TSAT/hepcidin ratios more resembling those observed in IRIDA patients. This evidence concerns the gene HAMP and IRIDA syndrome.