The human micro-vascular endothelial cell line of tumour angiogenesis (HMEC-1), if cultured in presence of MDA-MB-231 and BT-549 (ER−, human epidermal growth-factor receptor 2 negative (HER2−) breast ductal carcinoma) conditioned medium shows an enhanced angiogenic induction which is in turn significantly reduced upon Orai1 silencing, highlighting a role for Orai1 in Ca2+-mediated release of pro-angiogenic factors in triple negative breast cancer cell lines. This evidence concerns the gene ERBB2 and breast ductal adenocarcinoma.