The top 50 genes that were positively correlated with SPDL1 in colon cancer tissues were determined based on the TCGA data using the UALCAN web-based tool (Figure 4A), then verified for the expression profile in both colon and rectal cancers through GEPIA database, and confirmed to be significantly upregulated in tumors compared to normal tissues (data not shown). This evidence concerns the gene SPDL1 and rectal cancer.