With the aim to explore the effect of Calebin A on T-lymphocyte or TNF-α/TNF-β-triggered inflammation and NF-κB pathways activation as well as on tendon-specific transcription factor Scleraxis as potential targets, together with its underlying mode of action in vitro we created a multicellular tendinitis environment, consisting of fibroblasts, T-lymphocytes and tenocytes. The gene discussed is TNF; the disease is tendinitis.