While EVs from both 14 and 21 DIV cultures reduced slightly the secretion of the cytokines IFNγ and IL-1a (Supplementary Figure S3), only EVs from 14 DIV cultures decreased the levels of IL-6 (Supplementary Figure S3), a cytokine predominantly released in neurodegenerative diseases through EVs [37] in the CNS and involved in astrogliosis. This evidence concerns the gene IL1A and neurodegenerative disease.