TSC2 and fleck corneal dystrophy: Analysis of 40 individuals with FCD (previously screened and found to be negative for variants in MTOR gene) [52] identified two somatic TSC1 variants [c.610C>T (p.Arg204Cys) and c.64C>T (p.Arg22Trp)] in four individuals and one somatic TSC2 c.4639G>A (p.Val1547Ile) variant in one individual using targeted hybrid capture and PCR-based amplicon sequencing [58].