The main results of this study were that: (1) The expression of oncomiRs miR-21, miR-155, miR-221, and tumor suppressor miR-126 is dysregulated in PDAC cell lines compared to normal human ductal epithelial cells; (2) miR-21 moderates mRNA expression levels of key EMT markers, Wnt-11 expression, and cancer stem-like markers; (3) miR-21 KO significantly reduces cellular invasion capability of the two PDAC cell lines studied, indicating a role for miR-21 in cellular invasion capacity of PDAC in vitro. Here, WNT11 is linked to neoplasm.