Therefore, with the availability of assays for measuring plasma DAG and GOAT concentrations [23], we aimed to prospectively investigate the effect of a 12-week diet versus diet plus aerobic and resistance exercise programme on AG and DAG concentrations in adolescents with obesity, and to examine whether GOAT plays a causal role in DAG elevation with no change in AG concentrations. Here, MBOAT4 is linked to obesity due to melanocortin 4 receptor deficiency.