To uncover the role of GILZ in pulmonary inflammation and injury, we have established a murine mild model of ALI that exhibits intense neutrophil infiltration within 12–24 h of the intranasal challenge with LPS but resolves thereafter (Supplementary Figure S1A–C) and investigated the inflammatory response in GILZ deficient mice (GILZ−/−) compared to WT littermates (schematic experimental design in A). Here, TSC22D3 is linked to acute respiratory distress syndrome.