In addition, overexpression of mNGAL in 2L3 cells inhibited cell cyst enlargement (Figure 10) and occurred with a relatively lower secretion of NGAL (2.27 ng/mL) (Figure 7J) compared with the effect with high concentration of mNGAL (2 mg/mL) applied in previous 3D culture experiments (Figure 6). This evidence concerns the gene LCN2 and cyst.