Although we see stronger suppression of cyst enlargement in pN + LS group compared with pN − LS group in 3D culture, it is hard to see the better effect of pN + LS than pN − LS in p-AKT/AKT (Figure 8B), p-CREB/CREB (Figure 8C) and p62 (Figure 9D). This evidence concerns the gene CREB1 and cyst.