To explore the potential role of COX-2 in Drp1-mediated mitochondrial fission of HCC cells, we firstly identified the mRNA and protein expression of Drp1 and COX-2 in four HCC cell lines (QGY7703, HepG2, Huh7, and MHCC97H) and hepatic cell lines (L02 and QSG7701) by qRT-PCR and western blotting. This evidence concerns the gene DNM1L and hepatocellular carcinoma.