Interestingly, the depletion of PDK1 disrupted the cortical actin organization and cell motility of MTLn3 cells (highly metastatic mouse BC cells) in vitro and in vivo by regulating ROCK1 in a novel AKT-independent mechanism, in which ROCK1 was associated with RhoE in the absence of PDK1 [60]. This evidence concerns the gene AKT1 and breast cancer.