CD4 and colorectal cancer: Similarly, treatment of colorectal cancer-bearing mice with anti-CTLA-4 antibodies decreased Foxp3+/CD4+ Treg cells, but increased CD4+ T and CD8+ T cells and the expression levels of pro-inflammatory Th1/M1-related cytokines IFN-γ, IL-1α, IL-2, and IL-12 [52].