Lung (i.e., A549 and H1975 cell lines and NSCLC patients) and breast (i.e., MDA-MB-231 cell line and triple-negative breast cancer (TNBC) patients) samples were prepared for the isolation of total EVs (via differential ultracentrifugation) and EpCAM-positive EVs (via anti-EpCAM magnetic nanoparticles in CellSearch platform). The gene discussed is EPCAM; the disease is non-small cell lung carcinoma.