Moreover, overexpression of molecular lysine-specific histone demethylase 1 (LSD1), a histone-modifying enzyme responsible for demethylating histone H3 lysine 4 (H3K4) and histone H3 lysine 9 (H3K9), correlated with 5-FU resistance in human CRC specimens, and the small molecule LSD1 inhibitor ZY0511 combined with 5-FU synergistically suppressed CRC tumor proliferation and metastasis, both in vitro and in vivo, by targeting Wnt/β-catenin signaling and pyrimidine metabolic pathways [145]. This evidence concerns the gene KDM1A and neoplasm.