EGF and non-small cell lung carcinoma: As also outlined above, a kinase-dependent function elicited by EGFR at early endosomes was reported by Wei et al. [164], who also used wide-field immunofluorescence microscopy to show in HeLa cells and NSCLC cell lines (A549, HCC827, and H1975) that both EGF-bound wtEGFR and EGF-free activated EGFR mutants (L858R and D746–750) interact with Beclin 1 at early endosomes (Figure 5B).