AKT1 and non-small cell lung carcinoma: As an example, Han et al. [161] showed in NSCLC cell lines (A549, H1299, H292, H1650, and SK-MES-1) that gefitinib and erlotinib induce autophagy via inhibition of the PI3K/AKT/mTOR signalling pathway, and that the cytotoxicity of these TKIs was greatly enhanced after autophagy inhibition via chloroquine, a chemical that inhibits autophagic flux by decreasing autophagosome-lysosome fusion [162].