CD4 and neoplasm: Furthermore, CD4+ T cells and Tcytotoxic cell infiltration in the off-target tumour of the treated animals was increased by the sonodynamic therapy (parameters:f = 1 MHz, d.c. = 30%, prf = 100 Hz, P– = 0.48 MPa, mechanical index = 0.48, Sonication duration = 3.5 min during and 30 min after injection) [206].