ESCC showed the amplification of a region starting with CCND1 and ending around SHANK2 in 11q13.3, and another significant amplification in ESCC was from PIK3CA to SOX2 in 3q26.31, coupled with deletion of CDKN2A in 9p21.3. This evidence concerns the gene CDKN2A and esophageal squamous cell carcinoma.