Therefore, we constructed a xenograft mouse tumorigenesis model through orthotopic transplantation of human breast cancer cells either overexpressing ITIH5 (ΔpBK-ITIH5 clone #12) or lacking ITIH5 expression (ΔpBK-mock clone #1) [7] into the mammary fat pad of BALB/cnu/nu mice. Here, ITIH5 is linked to breast carcinoma.