To explore the expression levels of c-Myc and NKG2DL of SCLC cell lines, we firstly detected the expression of c-Myc, key transcription regulators of SCLC-N and SCLC-Y (ASCL1, NeuroD1, and YAP1) [24] and major NKG2DL (MICA, MICB, ULBP1-3) [18] in H2227, H446, H69, H524, and H196 cells by Western Blotting, flow cytometry, and qRT-PCR. The gene discussed is ASCL1; the disease is small cell lung carcinoma.