In the present study, it was found for the first time that LINC00525 knockdown decreased hypoxia-enhanced glycolysis, and overexpression of HIF-1α reversed the effect of LINC00525 knockdown on hypoxia-enhanced glycolysis in colorectal cancer, which suggests that LINC00525 promoted hypoxia-enhanced glycolysis, and this effect was HIF-1α dependent. The gene discussed is HIF1A; the disease is colorectal cancer.