Meanwhile, the BUB3 protein could also reverse the promotion effect of miR-664b-3p on the apoptosis of the HCT116 and DLD-1 while boosting the expression of Bcl-2 and inhibiting the production of Bax, caspase-3, and caspase-9 in colon cancer cells over-expressed of miR-664b-3p. The gene discussed is CASP9; the disease is malignant colon neoplasm.