Compared with the pcDNA3.1 group, the expressions of cancer stem cell-related genes ABCG2, NANOG, ALDH1, OCT4, and SOX2 were up-regulated by RSU1P2 or Tex10 overexpression, whereas down-regulated by let-7a overexpression; RSU1P2-induced upregulation of the above genes were suppressed by let-7a overexpression but aggravated by Tex10 overexpression; Furthermore, compared with the RSU1P2 + let-7a group, the expressions of ABCG2, NANOG, ALDH1, OCT4, and SOX2 were markedly elevated in the RSU1P2 + let-7a + Tex10 group (p < 0.05; Supplementary Figure S2F). The gene discussed is RSU1P2; the disease is cancer.