Furthermore, the percentages of CD4+ and CD8+ T cells in the splenic lymphocyte, tumour-infiltrating cell, and tumour-draining lymph node (TDLN) populations of RB, the RB-based mDC vaccine, and the RB-iDC group were significantly higher than those of the PBS control mice, with the CD8+ T cells exhibiting a more dramatic difference, especially in the RB-iDC group (Figure 6(f)). This evidence concerns the gene CD4 and retinoblastoma.